Function and Features
1.Activate the mucosal immune cascade reaction, cellular immunity and humoral immunity for the prevention and control of envelope virus, and establish the first and second defense lines of systemic immune system.
2.Activate the body's mucosal immunity, promote the activation and proliferation of B lymphocytes, increase the secretion of mucosal antibody SIgA, and block the invasion and adhesion of virus.
3.Activate humoral immunity, increase the secretion of humoral antibody IgG, inhibit virus adsorption and replication, and reduce the level of viremia.
4.Activate innate cellular immunity, activate macrophages, natural killer cells and T lymphocytes, induce the production of IFN - γ (interferon gamma), and block the immune escape of envelope virus.
5.It has the advantages of quick action.The beneficial function maintain a long time . It is convenient to use with no side effect and stress response.
How P2 activation blocks the immune escape of envelope virus?
1.P2 activates the first immune defense line of the body-- mucosal immune defense line：Lactobacillus plantarum LP-11 can stimulate intestinal mucosal immunity. Dendritic cell inducing peptide promotes the proliferation and activation of dendritic cells. IL-21 participates in the regulation of B cell proliferation, bone marrow progenitor cell proliferation and NK cell proliferation, differentiation and cytotoxicity. P2 activation induced immune cascade reaction in intestinal mucosa, which not only improved the phagocytosis of macrophages and the killing function of NK cells, but also promoted B cells to differentiate into plasma cells secreting sIgA, and significantly increased the content of sIgA in the surface layer of intestinal mucosa.
Secretory immunoglobulin A (sIgA) is the main antibody in mucosal exocrine fluid, which participates in local mucosal immunity and prevents pathogens from adhering to the cell surface by binding to envelope virus, thus playing an important role in local anti-infection. Compared with ordinary antibodies, it is a dimer of sIgA, which contains more antigen binding sites, so it has a stronger role in neutralizing pathogens and immune elimination.
Like a rope attached to the surface of the envelope virus, sIgA loses the ability to encode and express a variety of immune escape proteins to antagonize the host's innate immune system, as well as the ability to produce virulence and replication. It not only cannot infect monocytes such as macrophages, but also is quickly killed by macrophages, NK cells, T cells, interferon and so on.
Figure 1. The effect on the content of SIgA of mucosal antibody
Through the detection of mucosal antibody SIgA level, it was found that P2 activation can significantly increase the level of mucosal antibody SIgA.
2.P2 activates the body's second immune defense line-- cellular immune defense line: The dominant component activated by P2 stimulates T cells to differentiate and proliferate into memory T cells and effector T cells, in which memory T cells efficiently recognize the Px protein of the "killer arm and tool" of envelope virus, promote the rapid differentiation and production of effector T cells, enhance the cytotoxicity of effector T cells, and eliminate envelope virus in the early stage of proliferation.
P2 activation stimulates T lymphocytes to produce IFN-γ and other antiviral cytokines, which can be maintained in the body for a long time. IFN- γ has significant antiviral and immunomodulatory effects, eliminating intracellular capsule virus infection and enhancing cellular immune function. P2 activation can also induce the body to produce a variety of cytokines, inhibit the binding and invasion of envelope virus and host cells, positively regulate the host cell cycle, enhance cellular immune response, and block the immune escape of envelope virus.
Figure 2. The effect on the content of CD3 + CD4 + T lymphocytes in jejunum
P2 activation can significantly increase the number of CD3 + CD4 + T lymphocytes in PP node of jejunum.
Figure 3. The effect on the content of CD3 + CD4 + T lymphocytes in ileum
P2 activation can significantly increase the number of CD3 + CD4 + T lymphocytes in PP node of ileum.
Figure 4. The effect on the content of IL-2 in serum
P2 activation can significantly increase the content of IL-2 in serum2,after 14 days, 28 days and 42 days, they were 24%, 38% and 32% higher than those in the control group.
Figure 5.The effect on the content of IFN-γin serum
P2 activation can significantly increase the content of IFN-γin serum，after 14 days, 28 days and 42 days, they were 21%, 33% and 24% higher than those in the control group.
Figure6.The effect on the content of IgG in serum
Through the detection of serum IgG level, it was found that P2 activation can significantly increase the level of serum antibody IgG.
Evaluation data of P2 activation in vitro studied by Harbin Veterinary Institute,CAAS
Three major features of P2 activation products
1. Rapid reaction: Mucosal immune response quickly， and fully activated within 6 hours. And systemic immunity was stimulated by local immunity.
2. Stable and sustained potency: Key indicators such as SIgA, IFN-γ, DC cells, memory T cells and effector T cells, IL-21, intestinal flora structure were detected,which could be maintained for 30 to 45 days with one period, and some indicators could be maintained for 60 days.In order to ensure the effect of the product, one month product administration should be needed for one period .
3. Security: P2 activation is a Probiotics preparation, without any pathogenic factors, which can strengthen body resistance ，eliminat pathogenic factors and improve disease resistance. Prevent and control the cyst membrane virus. Relieve the immune suppression and promote the remarkable improvement of the immunity and resistance of pigs .Reduce morbidity and mortality and improve production performance.
P2 Activation product instructions
(1) Fields with low pressure of infection:
Daily usage, 1g/ sow or breeding boar/day and 0.5g/ fattening pig or piglet/day, once a day, for 7 consecutive days and once a month.
(2) Fields with medium pressure of infection (outbreaks occur 3 km away) :
Twice daily dosage, 2g/ sow or breeding boar/day and 0.5g/ fattening pig or piglet/day, once a day, for 7 consecutive days and once a month.
(3) Worst-hit areas and suspected early infection sites (outbreaks occur 3 km away) :
4 times daily dosage for the first time, 4g/ sow or breeding boar/day and 2g/ fattening pig or piglet/day, once a day and for 7 consecutive days. After one week, use twice daily dosage once again until the pigs stabilizes and then the dosage reaches daily dosage.
|Feeding method||Applicable object||Operation method||Note|
|Gavage||lactating pigs||Mix P2 and glucoseinjection, gavaged 1-2mL /head with syringe||When 1 bottle cannot be used up once, pour out the required P2 amount and put the remaining dry powder back into the refrigerator to avoid moisture absorption|
1. 1 bottle of P2+70mLofwater=100mL， use a syringe add to feed in the trough
2. Mix P2+water+feed
3.Add P2+water to the watering can, stir and spray evenly
1.Dilute P2 to liquid and add it to the sow feed tank for the best concentration
2.Liquid feedstuff feeding，eat in 2 hours or 1 meal
|Dry feedstuff||nursery pigs or growing pigs||Mix P2 and feed，eat in 2 hours or 1 meal||
1.Feed during feed control or early overhead trough
2.P2+ a small amount of flour，use a mixer to blend for 4-10 minutes
3.Dry mix is recommended for prevention, wet mix or drinking water is recommended for control
|Drinking water||Any stage||Add P2 to the water tank or sink, make up and drink according to the amount of drinking water in 2 hours||Control water in advance to reduce waste|